Analysis of lung surfactant phosphatidylcholine metabolism in transgenic mice using stable isotopes
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چکیده
منابع مشابه
Analysis of lung surfactant phosphatidylcholine metabolism in transgenic mice using stable isotopes.
Stable isotope labelling of lipid precursors coupled with mass spectrometry-based lipidomic analyses and determination of isotope enrichment in substrate, intermediate and product pools provide the parameters needed to determine absolute flux rates through lipid pathways in vivo. Here, as an illustration of the power of such analyses we investigated lung phosphatidylcholine (PC) synthesis in Su...
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An investigation of the effect of change of total COP concentration from 7 to 43 mM at pH 7.35 in the medium perfusing isolated rat lungs on [U-I4C]glucose incorporation into lung phospholipids has been carried out. The incorporation of [U-14C]glucose into phosphatidylcholine and phosphatidylglycerol of the surfactant fraction and of the remaining lung tissue (residual fraction) was observed. I...
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An investigation of the effect of change of total CO(2) concentration from 7 to 43 mM at pH 7.35 in the medium perfusing isolated rat lungs on [U-(14)C]glucose incorporation into lung phospholipids has been carried out. The incorporation of [U-(14)C]glucose into phosphatidylcholine and phosphatidylglycerol of the surfactant fraction and of the remaining lung tissue (residual fraction) was obser...
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Pulmonary surfactant prevents alveolar collapse and contributes to airway patency by reducing surface tension. Although alveolar surfactant, consisting mainly of phospholipids (PL) together with neutral lipids and surfactant-specific proteins, originates from type II pneumocytes, the contribution of airway epithelia to the PL fraction of conductive airway surfactant is still debated. We, theref...
متن کاملSurfactant disaturated-phosphatidylcholine kinetics in acute respiratory distress syndrome by stable isotopes and a two compartment model
BACKGROUND In patients with acute respiratory distress syndrome (ARDS), it is well known that only part of the lungs is aerated and surfactant function is impaired, but the extent of lung damage and changes in surfactant turnover remain unclear. The objective of the study was to evaluate surfactant disaturated-phosphatidylcholine turnover in patients with ARDS using stable isotopes. METHODS W...
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ژورنال
عنوان ژورنال: Chemistry and Physics of Lipids
سال: 2011
ISSN: 0009-3084
DOI: 10.1016/j.chemphyslip.2011.04.004